Detection and molecular characterization of phytoplasmas based on 16s rDNA gene region by phylogenetic and in silico RFLP analysis of local grapevine cultivars in ?anl?urfa and Ad?yaman

During the autumn seasons of 2016-2019, several surveys were carried out in grapevine (Vitis vinifera L.) cultivation areas ?anl?urfa and Ad?yaman provinces South-eastern part Turkey, to identify plants exhibiting phytoplasma-like symptoms including yellowing, small leaf formation, chlorosis, short internodes, severe redness inward curling detect characterize causal agent responsible for these symptoms. Purified DNA obtained from collected samples was examined highly conserved phytoplasma 16S rDNA gene via nested-PCR with universal phytoplasma-specific primer sets. amplification nested-PCR/RFLP analyses some restriction enzymes confirmed suspected correlations between disease observed presence samples. F2n/R2 amplicons strains showed 99.99% homology each other 99% partial sequences belonging groups deposited GeneBank database. Sequencing phylogenetic analysis revealed that isolates have sequence identity ‘Candidatus solani’ (16SrXII-A) ‘Ca.P. asteris’ (16SrI-B). According our knowledge, results this study is first report relationship phytoplasmas infecting different cultivars based on gene. Genetic diversity genes than pathogen causing infections under study.